Transformation innovations
A major focus of the laboratory is on developing novel methods for stimulating the regeneration of transgenic plants using a variety of “developmental (DEV) genes” that directly stimulate cellular growth and meristem development. We are particularly interested in genes that can stimulate “in planta” (greenhouse-based) transformation as well as those helpful in vitro (aseptic growth media), with emphasis on poplars, eucalypts, and hops.
Figure: Accelerated development of transgenic shoots in poplar using DEV genes from Agrobacterium. The genes were used in an altruistic manner (cotransformation of DEV and marker genes). Shown are false-color images that show transgenic tissues based on hyperspectral imaging of green-fluorescent-protein expression.